Sauer:Making yeg plates

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YEG medium: 0.5% yeast extract

1% NaCl

0.4% glucose

supplement with 16 mM p-Cl-Phe for yeg-pcp.


pcp is not soluble before you autoclave.

    • DO NOT AUTOCLAVE THE GLUCOSE, ADD AFTER FROM STERILE STOCK**

YEG-agar: 5 g yeast extract

10 g NaCl

2 g D,L-p-Cl-Phe

15 g agar

1 L H20

AUTOCLAVE

add 0.4% glucose, antibiotics

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