Revised by Kenneth Eum on 11-15-2011
Cell Media with Selection Agents (Zeocin and Blasticidin) for Kv2.1 TREx CHO cell line
F12 from Gibco, 10% FBS, 1% Penicillin/Streptomycin, 1/10,000 Blasticidin, 1/4,000 Zeocin
Blasticidin = 10mg/ml stock
Zeocin = 100mg/ml stock
- Obtain a 50mL conical tube and label as:
- “Ham’s F-12 Media + 10% FBS + 1% Pen/Strep + 1ug/ml blast + 25ug/ml zeo”
- Include date made and initials
- Add 44.5mL of Ham’s F-12 Media to the labeled 50mL conical tube
- Add 5mL of FBS
- Add 500µL of Pen Strep
- Add 5µl of blasticidin (Final Concentration (1 µg/ml))
- Add 12.5µl of zeocin (Final Concentration (25 µg/ml))
- Close the conical tube tightly and store in 4°C