SIL082506-Construction of pSB1AK3-J23074
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[RA2 Promoter][RBS][TraGr][TT]
Digest PCR product of pSB1A2-J23038 with G00101 and ca641R (BSAI/SpeI, Neb2, ~1600 )
Paste into pSB1AK3-J23026 (XbaI/BsaI, Neb2, Large 5229)
Product is pSB1AK3-J23074.
JCAnderson 20:11, 16 September 2006 (EDT)Revised construction:
Construction of pSB1A2-J23074
[Pcon][stRBS][traG][TT]
Digest pSB1AK3-J23026 . . . . . . . . (XbaI/PstI, small-2072 bp)
Digest pBca1020-J23038 . . . . . . . .(SpeI/PstI, large-2121 bp)
Product is pSB1A2-J23074
