SIL071106-Construction of pSB1A2-J23011
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[TraMf]
PCR JL5/JL6 on Olambda (432bp, EcoRI/SpeI, Neb2)
Paste into pSB1A2-I13521 (EcoRI/SpeI, Neb2, Large 2062)
Product is pSB1A2-J23011
JL5 Forward EcoRI oligo to bioBrick TraM:
CCTAGGAATTCGCGGCCGCTTCTAGatggctaaggtgaacctgtatatcagc
JL6 Reverse SpeI oligo to bioBrick TraM:
gacttACTAGTgtcaaatttcgtttattcatc
