Serine Integrase Directional Recombination
The natural function of the large serine integrases (such as φC31 Integrase) is to integrate circular DNA into a bacterial host chromosome, by recombining attP with attB.
Recombination is highly directional. In the presence of the integrase alone, attP recombines efficiently with attB to form attL and attR which do not recombine further. However, in the presence of a recombination directionality factor (RDF) attL and attR recombine to re-form attP and attB.
This directional recombination can be used for a variety Synthetic Biology Applications
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