SBB09 Construct44

From OpenWetWare

Jump to: navigation, search
Construction of Polystyrene-Binding Peptide 

 Wobble Ons003F/Ons004R           (67bp, EcoRI/BamHI)
 Sub into pBca9495AK-Bca1144#5    (EcoRI/BamHI, 3171+910, L)
 Product is pBca9495AK-M10021     {<Poly_bind>}
 ----
 Ons003F   Forward construction of Poly_bind basic part     ccataGAATTCatgAGATCTttcaaattctggctatacgaacatgtaatacggggg
 Ons004R   Reverse construction of Poly_bind basic part     ctgatGGATCCcccccgtattacatgttcgt


 JCA:  Your construction file is "correct", but in general repeats on the 3' end of an oligo are bad news, particularly if they are G's since 4G's makes a particular secondary structure in and of itself.  See revision:
Construction of Polystyrene-Binding Peptide 

 Wobble Ons003F/Ons004R           (67bp, EcoRI/BamHI)
 Sub into pBca9495AK-Bca1144#5    (EcoRI/BamHI, 3171+910, L)
 Product is pBca9495AK-M10021     {<Poly_bind>}
 ----
 Ons003F   Forward construction of Poly_bind basic part     ccataGAATTCatgAGATCTttcaaattctggctatacgaacatg
 Ons004R   Reverse construction of Poly_bind basic part     ctgatGGATCCcccccgtattacatgttcgtatagccagaat
Personal tools