SBB09 Construct42

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Construction of Ice Nucleation Repeats (INP repeats) Basic Part
PCR Oeh001F/Oeh002R on pBca1256-Bca1346		{2296bp, EcoRI/BamHI}
Sub into pBca9145-Bca1144#5			{EcoRI/BamHI, 3171+910,L}
Product is pBca9145-Beh001			{<INP repeats>}
---
eh001F Forward EcoRI oligo for <INP repeats>
gtcagGAATTCatgAGATCTaaccacagtgatctaattgc
eh001R Reverse BamHI oligo for <INP repeats>
gtcagGGATCCactgtcctgcccggccgttg
 JCA:  the pcr is right, but you have it in the wrong vector.  Also, use
 your M numbers.  See revised:
Construction of Ice Nucleation Repeats (INP repeats) Basic Part
PCR Oeh001F/Oeh002R on pBca1256-Bca1346		(2296bp, EcoRI/BamHI)
Sub into pBca9495AK-Bca1144#5			(EcoRI/BamHI, L)
Product is pBca9495AK-M10048      {<INP repeats>}
---
eh001F Forward EcoRI oligo for <INP repeats>
gtcagGAATTCatgAGATCTaaccacagtgatctaattgc
eh001R Reverse BamHI oligo for <INP repeats>
gtcagGGATCCactgtcctgcccggccgttg
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