SBB09 Construct38

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Construction of <a~Inv-short> basic part Bcr0001

PCR Ocr0001-F/Ocr0001-R on pAC-TetInvdBg (2735 bp, EcoRI/BamHI)
Sub into pBca9495CA-Bca1144#5 (EcoRI/BamHI, 3039+910, L)
Product is pBca9495CA-Bcr0001  {a~Inv-short>}
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Ocr0001-F       forward oligo for biobricking of {a~Inv-short>}
ccaaaGAATTCatgAGATCTaattatgatggttttccagccaatcagtg
Ocr0001-R       reverse oligo for biobricking of  {a~Inv-short>}
ccaaaGGATCCactggataccagcgatctgccgc
 JCA:  Ocr0001-F doesn't need to be quite so long:
Construction of <a~Inv-short> basic part Bcr0001

PCR Ocr0001-F/Ocr0001-R on pAC-TetInvdBg (2735 bp, EcoRI/BamHI)
Sub into pBca9495CA-Bca1144#5 (EcoRI/BamHI, 3039+910, L)
Product is pBca9495CA-M10030  {a~Inv-short>}
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Ocr0001-F       forward oligo for biobricking of {a~Inv-short>}
ccaaaGAATTCatgAGATCTAATTATGATGGTTTTCCAGC
Ocr0001-R       reverse oligo for biobricking of  {a~Inv-short>}
ccaaaGGATCCactggataccagcgatctgccgc
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