SBB09 Construct21

From OpenWetWare

Jump to: navigation, search
Wobble Osc0028F/Osc0029R           (136bp, EcoRI/BamHI)
Sub into pBca9495AK-Bca1144#5    (EcoRI/BamHI, 3171+910, L)
Product is pBca9495AK-M10053     {passenger protein}
----
Osc0028F   Forward construction of passenger protein basic part
TCCGGgaattcATTagatctGTTAAAGAACTGGAAGACAAAAACGAAGAACTGCTGTCTGAAATCTACCACCTGGACAACGAAG
Osc0029R   Reverse construction of passenger protein basic part
CTGGTggatccGCAACCACCACGTTCACCAACCAGTTTTTTCAGACGAGCAACTTCGTTGTCCAGGTGGTAG
 JCA:  Put atg between eco and bam; renamed short description.  Revised below:
Wobble Osc0028F/Osc0029R           (136bp, EcoRI/BamHI)
Sub into pBca9495AK-Bca1144#5      (EcoRI/BamHI, 3171+910, L)
Product is pBca9495AK-M10053    {<EILD>}
----
Osc0028F   Forward construction of Leucine zipper EILD
TCCGGgaattcATGagatctGTTAAAGAACTGGAAGACAAAAACGAAGAACTGCTGTCTGAAATCTACCACCTGGACAACGAAG
Osc0029R   Reverse construction of Leucine zipper EILD
CTGGTggatccGCAACCACCACGTTCACCAACCAGTTTTTTCAGACGAGCAACTTCGTTGTCCAGGTGGTAG
Personal tools