SBB09 Construct20

From OpenWetWare

Jump to: navigation, search
Construction of ehaB Basic Part 
PCR Osc0030F/Osc0031R on ehaB gene           (1257bp, EcoRI/BamHI)
Sub into pBca9495KC-Bca1144#5               (EcoRI/BamHI, 2974+910, L)
Product is pBca9495KC-M10052  {<ehaB!]
-------------------------------------
Osc0030F  Forward Biobricking of ehaB  
ggcctGAATTCattAGATCTggcttaatcgcaatgaactct
Osc0031R  Reverse Biobricking of ehaB
gtaccGGATCCttaccaggtatatttaacac
 JCA:  This might go past the "true" stop, but should still work either way
Personal tools