SBB09 Construct15
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Construction of {<inv>} basic part from PAC-TetInvdBg PCR Odmd001F and Odmd002R on PAC-TetInvdBg (2593bp, EcoRI/BamHI) Sub into pBca9495CA-Bca1144#5 (EcoRI/BamHI, 3039+910, L) Product is pBca9495CA-M10050 {<inv>} ---------------------------- Odmd001F Forward Oligo for Invasin (refactored, long) ccataGAATTCatgAGATCTgctggggcttcagaaaaa Odmd002R Reverse Oligo for Invasin (refactored, long) ccaaaGGATCCactggataccagcgatctgccg
JCA: The short description needs to be a little more specific. The five A's at the end of Odmd001F are a little funky. I changed that oligo a little:
Construction of {<inv>} basic part from PAC-TetInvdBg PCR Odmd001F and Odmd002R on PAC-TetInvdBg (2593bp, EcoRI/BamHI) Sub into pBca9495CA-Bca1144#5 (EcoRI/BamHI, 3039+910, L) Product is pBca9495CA-M10050 {<inv_long/ref.>} ---------------------------- Odmd001F Forward Oligo for Invasin (refactored, long) ccataGAATTCatgAGATCTGCTGGGGCTTCAGAAAAATATGATGC Odmd002R Reverse Oligo for Invasin (refactored, long) ccaaaGGATCCactggataccagcgatctgccg