SBB09 Construct11

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Construction of Tsh Autotransporter

PCR Odd001F/Odd002R on E. Coli plasmid pAPEC-1 (AF218073)	(1603 bp, EcoRI/BamHI)
Sub into pBca9495KC-Bca1144#5					(EcoRI/BamHI, 2974+910 bp, L)
Product is pBca9495KC-M10040					{<TshA!}
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Odd001F	Forward EcoRI oligo for TshA	ccataGAATTCatgAGATCTacacttttacctgtatacg
Odd002R	Reverse BamHI oligo for TshA	ctgatGGATCCtcagaatgaataacgaatattagcg

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