SBB09 Construct10

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Bglbricking of espP(beta)
PCR osn0001/osn0002 on Escherichia coli O157:H7 str. Sakai           (1381 bp, EcoRI/BamHI)
Sub into pBca9495KC-Bca1144                                          (EcoRI/BamHI, 2974+910, L)
Product is pBca9495KC-M10044	{<espP(beta)!}
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Osn0001-F  Forward Bglbricking of espP of Escherichia coli O157:H7 str. Sakai    ccagtGAATTCatgAGATCTgccccgtcagcatctgccac
Osn0002-R  Reverse Bglbricking of espP of Escherichia coli O157:H7 str. Sakai    gcagtGGATCCtcagaacgagtaacggaaattag