Richard Lab:IC eluents

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Contents

System 1

Overview

IC System 1 consists of the ICEAS1 guard and analytical columns, a 750μL reaction coil, a UV detector (for organic acid detection), and an electrochemical detector with platinum electrode (for alcohol detection). The 4 eluent bottles are configured as listed here, with eluents A, B, and C requiring degassing: A = 100 mM methanesulfonic acid (2 L), B = 100 mM methanesulfonic acid (2 L), C = nanopure water (2 L), D = acetonitrile (1 L). Eluents A and B are used for running samples, while eluents C and D are available for system cleaning. A procedure for preparing eluents A and B is given below.

Materials

Supplies

  • 2000-mL volumetric flask
  • funnel
  • eluent bottles
  • 25 mL pipet
  • vacuum pump
  • 2000 mL vacuum flask
  • stir plate and stir bar

Chemicals

  • methanesulfonic acid (fume hood cabinet, acids)
  • acetonitrile (fume hood cabinet, flammables)

Procedure

  1. Fill vacuum flask with approximately 2 L nanopure water.
  2. Place on stir plate (~400 rpm) and connect to vacuum pump to degas. Let degas for ~10 minutes. While degassing:
    1. Disconnect helium going to eluent bottle headspace (easiest way to do this is by disconnecting tubing at pressure regulator on top of IC detector compartment).
    2. Disconnect eluent bottle. Pour any remaining eluent into waste container (or water can be poured directly into sink).
    3. Rinse bottle 3 x with nanopure water.
  1. Pour slightly less than 2 L degassed water (below flask neck) into volumetric flask.
  2. Pipet 13 mL methaneculfonic acid into volumetric flask (submerge pipet tip).
  3. Bring final volume to 2 L.
  4. Cap and invert volumetric flask gently to mix.
  5. Add prepared 100 mM methanesulfonic acid to eluent bottle. Reconnect bottle to lid and to helium. Check that endline filter is in good, clean condition. Change as needed.
  6. All new eluents must be primed. This should be done one eluent at a time, ending with the eluent that will be used.
    1. Open priming knob on pump 1 by turning ½ turn (see illustration on pump compartment door).
    2. On computer, set eluent to be primed to 100%. Start priming at 4 mL/min for 300 s. This will draw ~ 20 mL through pump.
    3. While priming, observe that eluent is flowing from eluent bottle and bubbles are being removed through waste line on pump.
  7. When priming is complete, turn on pump motor to desired flow rate. Then close priming knob so it is finger tight. Monitor pressure increase and stability.

Notes

References

Contact

  • Who has experience with this protocol?

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System 2

Overview

Replace this sentence with a brief description of the protocol and its goal.

Materials

List reagents, supplies and equipment necessary to perform the protocol here. For those materials which have their own OWW pages, link to that page. Alternatively, links to the suppliers' page on that material are also appropriate.

  • supply 1 (i.e. tubes of a certain size? spreaders?)
  • reagent 1
  • X μL reagent 2
    • component A (reagent 2 is made up of multiple components)
    • component B
  • equipment 1
  • equipment 2

Procedure

  1. Step 1
  2. Step 2
    • Step 2 has some additional information that goes with it. i.e. Keep at 4°C.
  3. Step 3
    1. Step 3 has multiple sub-steps within it.
    2. Enumerate each of those.

Notes

References

Contact

  • Who has experience with this protocol?

Back to Protocols








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