Restriction digest/Buffers

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Contents

New England Biolabs

1X NEBuffer 1

  • 10 mM Bis-Tris-Propane-HCl
  • 10 mM MgCl2
  • 1 mM Dithiothreitol
  • pH 7.0 @ 25°C

1X NEBuffer 2

  • 50 mM NaCl
  • 10 mM Tris-HCl
  • 10 mM MgCl2
  • 1 mM Dithiothreitol
  • pH 7.9 @ 25°C

1X NEBuffer 3

  • 100 mM NaCl
  • 50 mM Tris-HCl
  • 10 mM MgCl2
  • 1 mM Dithiothreitol
  • pH 7.9 @ 25°C

1X NEBuffer 4

  • 50 mM potassium acetate
  • 20 mM Tris-acetate
  • 10 mM Magnesium Acetate
  • 1 mM Dithiothreitol
  • pH 7.9 @ 25°C
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