Registry/Measurement kit/Notebook/2007-7-7

• Got growth on:
  • T9002
    • 3K3 (from both sequential and double digests)
  • I2055
    • 1AK3
  • E0240
    • 3K3
    • 1AK3 (2 colonies)

• Will colony PCR
• Gel of I2055 (with promoter) looks ok, will sequence to make sure.

• PCR cleaned I2055
• Double M/N digest in buffer 2

• I2056 doesn't seem to grow in o.n. amp/tet culture...trying with just tet.

• Ligating T9002 into 1AK3