- Began PCR to remove BioBrick restriction sites from T9002 and E0240
Sequencing of I2055
- 2µL of 1.6µM primers
- needed 200-500 ng of DNA, or about 1µL
- add water to 12µL.
- tube 1: forward primers; tube 2: reverse
RFP-based device construction
- started digests of backbones 1AK3, 1AT3, 1AC3, and parts B0032, R0040, and J04650