Protocol name dnastar

Overview

DNA Star is a bioinformatics software that can facilitate primer design, sequence verification, and

Materials

DNA Star software

• SeqBuilder
• Primer Select
• EditSeq
• SeqMan

Procedure

• Create sequence file

1. Copy and paste expected sequence from PlasmoDB into an old Word file ('97-'03). Save file. Transfer to front computer via flash drive.
2. Open file. Open EditSeq > New Sequence > DNA. Copy and paste from Word to open window. Save file.

• Verify sequence

1. Open SeqMan > Add sequence > Select forward and reverse sequences from shipment + EditSeq file of actual sequence > Assemble
2. Window will pop up:

ONE contig file = good MORE THAN ONE contig file = not good

1. Double-click on a contig file to review sequence alignment.
2. Red = mutation

No red = no mutation

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

1. List troubleshooting tips here.
2. You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
3. Anecdotal observations that might be of use to others can also be posted here.

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Contact

• Who has experience with this protocol?

or instead, discuss this protocol.