Penn State University 2006:lbplates

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LB Plates

(per/L H2O)

Procedure

1. Weigh and add to H2O in large Erlenmeyer:

  • 10 g Bacto-tryptone
  • 5 g Bacto-yeast extract
  • 10 g NaCl
  • 15 g Bacto-agar

2. Autoclave on slow exhaust cycle 25 min

3. Cool in tap-water-bath 10 min, then place in 45ºC water bath. (in sterile hood)

4. When media has cooled to ~45ºC (comfortable to hold), add appropriate antibiotic.

5. Pour plates (~25 mL/plate)

6. Allow to solidify & store in bag in cold-room

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