Pelling:Protocols/Protozoan Media Formulations

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Chalkley's Solution (CH) 100mL 1000X Stock Solution: 10g NaCl 0.4g KCl 0.6 CaCl2 Autoclave in 100mL of dH2O Prescott's & James's Solution (PJ) 100mL 1000X Stock Solution: 0.43g CaCl2-2H2O 0.16g KCl 0.51g K2HPO4 0.28g MgSO4-7H2O Autoclave in 100mL of dH2O Wheat Grains Soft Wheat Berries were obtained from The Wheat Berry (206 Main Street, Ottawa, ON K1S1C6, 613-235-7580). Wheat grains are boiled in dH2O for 10 minutes, cooled and stored in the fridge. Unboiled wheat should be keep at room temperature in a Duran bottle. Hay Media 400mL 4X Stock Solution Timothy Hay is obtained from Ritchie Feed & Seed. Cut up 6g of Timothy Hay into 1" pieces. Add to 400mL of dH2O in a 500mL Duran bottle. Autoclave and let cool to room temp. Filter sterilize to remove hay and particulates, store in fridge.