Pelling:Lab Supplies/Cell Lines

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Cell Line Species and Type Notes
HeLa Human Epithelial Monolayer line, Passage at 70-80%
HeLa-FP Human Epithelial Monolayer line, Passage at 70-80%, Requires 400 ng/mL Puromycin and 600 ug/mL G418, Stable expression of Actin-mRFP and Tubulin-GFP
HEK293 Human Embryonic Kidney Passage at 70-80%
HFF Human Foreskin Fibroblast Contact inhibited monolayer, Passage at 70-80%
SAOS2 Human Osteoblast-like Epithelial Monolayer line, Passage at 70-80%
NIH3T3 Mouse Fibroblast Passage at 70-80%, Do not allow to become confluent as fusion and differentiation will occur
HL-1 Mouse Cardiomyocyte BEWARE: Requires very specific culture/serum and growth conditions talk to Dr. Pelling first!
C2C12 Mouse Myoblast Passage at 70-80%, do not allow to become confluent as fusion and differentiation will occur
IMCD Mouse Inner Medullary Collecting Duct Epithelial Monolayer Line, DMEM:F12, 10%FBS, Passage at 70-80%
CHO Chinese Hamster Ovary Epithelial F12, 10%FBS, 1% Non-Essential Amino Acids, Passage at 70-80%
MDCK Madin Darby Canine Kidney Epithelial Passage at 70-80%, Do not allow to become over confluent, Cells detach slowly from surface
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