NanoBio: BG-11 plates
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BG-11 plates
- For 1 L, or ~2 sleeves of plates, place in a 2L flask:
- 10 mL 1 M TES pH 8.2 (Sigma catalog #)
- 3 g sodium thiosulfate (Sigma, catalog #)
- 15 g Bacto-agar (catalog #)
- 20 mL 50x BG-11 media (Sigma catalog #)
- 980 mL distilled water
- a stirring bar
- Autoclave at 121C for 20 minutes on the liquid cycle.
