Michael Eisen Lab:Reagents:PBS

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Phosphate Buffered Saline

Contents

Usage

PBS is an isotonic buffer for cells.

Preparation

Purchase in Solution

This should be used as a last resort.

  • PBS is available as 10X Solution from the Stanley Hall stock room

Prepare from tablets

  • PBS is ideally prepared from PBS tablets <ref> Sigma P4417 </ref> or constituent salts (see wikipedia page).
    • Sterilize solution with sterile filter.

1X PBS

1x PBS is 137 mM NaCl, 12 mM Phosphate, 2.7 mM KCl, pH 7.4

10X PBS Stock Solution

  1. Combine the following:
    • 80 g NaCl
    • 2 g KCl
    • 14.4 g Na2HPO4 (dibasic anhydrous) OR 18.1 g Na2HPO4 · 2H2O (dibasic dihydrate) OR 27.2 g Na2HPO4 · 7H2O (dibasic heptahydrate)
    • 2.4 g KH2PO4 (monobasic anhydrous)
    • 800 mL distilled H2O
  2. Adjust pH to 7.4 with HCl
  3. Add H2O to 1L
  4. Autoclave for 20 minutes on liquid cycle or sterile filter. Store at RT.

Storage

  • PBS tablets on chemical shelf
  • PBS stock solutions on benchtops


Wikipedia.org PBS Page

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