McClean:YNB4

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Contents

Overview

Yeast nitrogen base liquid without amino acids with glucose. This protocol leaves NO ROOM for additives.

Reagents

  • Yeast Nitrogen Base (without amino acids)
  • MilliQ H2O
  • Dextrose

Protocol

  • Begin with approximatey 800mls of MiliQ H2O
  • Weigh out and add ingredients with constant stirring:
    • Yeast Nitrogen base (without amino acids) 6.7g
  • Bring up to 900mls with MiliQ H2O
  • Weight out 20g of glucose/dextrose and add to 100mls of MiliQ water
  • Autoclave the YNB and glucose separately.
  • Combine YNB with glucose solution

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

  1. List troubleshooting tips here.
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References

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