Matt Gethers/OWW Syntax
From OpenWetWare
Contents |
OWW Syntax
The Official Syntax
My Short Cuts
Bibliography
- Arias-Barrau E, Olivera ER, Luengo JM, Fernández C, Galán B, García JL, Díaz E, and Miñambres B. . pmid:15262943.
- Fuangthong M, Sallabhan R, Atichartpongkul S, Rangkadilok N, Sriprang R, Satayavivad J, and Mongkolsuk S. . pmid:17957353.
- Fuangthong M and Helmann JD. . pmid:14563870.
Files
Thumbnails
Linking to Pages in OWW
The GFP and Mcherry scaffolds I'm using were constructed by Heather Keller of the Endy Lab.
Linking to Pages outside of OWW
Tables
| Approach | Pros | Cons | References |
| DNA (5')/MLS Conjugate | 24mer oligo has been successfully imported into isolated yeast mitochondria using this method (Vestweber et al); Reagents are readily accessible and cheap. | It is unknown if the conjugate (specifically the peptide portion) can get across the yeast cell membrane; Crosslinking of DNA to peptide may inhibit expression and recombination. | [4] |
| DNA (Internal)/MLS Conjugate | ~300 bp strand of DNA successfully imported into isolated rat mitochondria (Siebel et al). | Experiment performed with mammalian mitochondria;Crosslinking of DNA to peptide may inhibit expression and recombination. | [5] |
| DNA/MLS Conjugate (Crosslinking to peptide done in vivo) | Resolves problem of getting peptide across cell membrane. | Singly-bound crosslinker is likely bind any sulfhydryl group it encounters during transformation. | Natalie's Suggestion |
| DNA/MLS Conjugate with Cytosolic leading signal (Endocytosis) | Resolves problem of getting the conjugate across the yeast cell membrane without attempting to crosslink in vivo | Digestion or rejection during endocytosis? Formation of lipid membrane around conjugate? | Drew's Suggestion |
