The Official Syntax
My Short Cuts
- Arias-Barrau E, Olivera ER, Luengo JM, Fernández C, Galán B, García JL, Díaz E, and Miñambres B. . pmid:15262943.
- Fuangthong M, Sallabhan R, Atichartpongkul S, Rangkadilok N, Sriprang R, Satayavivad J, and Mongkolsuk S. . pmid:17957353.
- Fuangthong M and Helmann JD. . pmid:14563870.
Scaffold Map of High Copy Vector (Genbank Files to Follow)
Linking to Pages in OWW
The GFP and Mcherry scaffolds I'm using were constructed by Heather Keller of the Endy Lab.
Linking to Pages outside of OWW
lambda pL promoter (BBaR0011)
|DNA (5')/MLS Conjugate||24mer oligo has been successfully imported into isolated yeast mitochondria using this method (Vestweber et al); Reagents are readily accessible and cheap.||It is unknown if the conjugate (specifically the peptide portion) can get across the yeast cell membrane; Crosslinking of DNA to peptide may inhibit expression and recombination.||
|DNA (Internal)/MLS Conjugate||~300 bp strand of DNA successfully imported into isolated rat mitochondria (Siebel et al).||Experiment performed with mammalian mitochondria;Crosslinking of DNA to peptide may inhibit expression and recombination.||
|DNA/MLS Conjugate (Crosslinking to peptide done in vivo)||Resolves problem of getting peptide across cell membrane.||Singly-bound crosslinker is likely bind any sulfhydryl group it encounters during transformation.||Natalie's Suggestion
|DNA/MLS Conjugate with Cytosolic leading signal (Endocytosis)||Resolves problem of getting the conjugate across the yeast cell membrane without attempting to crosslink in vivo||Digestion or rejection during endocytosis? Formation of lipid membrane around conjugate?||Drew's Suggestion