Matt Gethers/CRI, Thailand/Labwork/PCRs/Screening for Presence of Lox/Gen Cassette in pKn004/8.5.08 Master mix
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8.5.08 PCR Master
| Reagent | Volume/Amount |
| Colony Suspension | 8.5 μl, but don't add to master |
| Taq | 0.85 μl |
| M13 for | 6.8 μl 1 μM Stock |
| BT1188 | 6.8 μl 1 μM Stock |
| dNTPs | 1.7 μl 10 mM Stock |
| DMSO | 4.25 μl |
| Buffer | 8.5 μl |
| Mg2+ | 4.25 μl |
| dH20 | 43.35 μl |
| Total | 50 μl |
Aliquotted 9 μl to each of 8 tubes, 7 colony PCRs, 1 negative control using 0.5 μl pKn004.P1. Added 1 μl colony suspension to the rest.
