Matt Gethers/CRI, Thailand/Labwork/PCRs/Screening for Presence and Directionality of HmgA Upstream Fragment in pUC18/Gel results for 7.15.08 PCR

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7.15.08

Lane 1 Lane 2 Lane 3 Lane 4 Lane 5 Lane 6 Lane 7 Lane 8 Lane 9 Lane 10 Lane 11 Lane 12 Lane 13 Lane 14 Lane 15 Lane 16
5 μl λ DNA Ladder 7.14.08 pKn003.L2 transformation colony PCR product #1 7.14.08 pKn003.L2 transformation colony PCR product #2 7.14.08 pKn003.L2 transformation colony PCR product #3 7.14.08 pKn003.L2 transformation colony PCR product #4 7.14.08 pKn003.L2 transformation colony PCR product #5 7.14.08 pKn003.L2 transformation colony PCR product #6 7.14.08 pKn003.L2 transformation colony PCR product #7 Space 7.14.08 pKn004.L2 transformation colony PCR product #1 7.14.08 pKn004.L2 transformation colony PCR product #2 7.14.08 pKn004.L2 transformation colony PCR product #3 7.14.08 pKn004.L2 transformation colony PCR product #4 7.14.08 pKn004.L2 transformation colony PCR product #5 7.14.08 pKn004.L2 transformation colony PCR product #6 7.14.08 pKn004.L2 transformation colony PCR product #7 pKn002.P1 negative control

100 volts, 30 minutes, 1% gel, 20 minutes staining. All lanes showed a product at ~500 bp again, however colony 5 of the pKn003.L2 transformants gave product at a little lower than 1300 bp, so I've taken that to make a glycerol and sequence. Everything failed on the pKn004 again - I have to fix the digest.

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