Matt Gethers/CRI, Thailand/Labwork/PCRs/PCR Supermix Template
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PCR Supermix Template
Rxn Mix
Reagent | Volume/Amount |
DNA Template | 1.0 μl from Colony Suspension |
1.2 μl 1 μM Stock | |
1.2 μl 1 μM Stock | |
2x PCR Master | 7.5 μl |
H20 | 4.1 μl |
Total | 15 μl |
Rxn Conditions
Annealing Temperature: oC
Cycle (Taq)
Step | Temperature | Duration | Notes |
Initial Denaturation | 95oC | 2 minutes | |
Repeat Cyclic Steps 35x | |||
Cyclic Denaturation | 94oC | 30 Seconds | |
Cyclic Annealing | oC | 30 Seconds | |
Cyclic Extension | 72oC | minute | |
Repeat Cyclic Steps 35x | |||
Final Extension | 72oC | 10 minutes |