Matt Gethers/CRI, Thailand/Labwork/HmgR Isolation Protocol/8.9.08 Gel results
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8.9.08 SDS-PAGE Induction Assay
Lane 1 | Lane 2 | Lane 3 | Lane 4 | Lane 5 |
5 μl Unstained protein marker | 5 μl P7/-IPTG | 5 μl P7/+IPTG | 5 μl P8/-IPTG | 5 μl P8/+IPTG |
Ran at 200 V for 1 hour, then stained with 0.3% Coomassie for 30 minutes. While the +IPTG lanes appear to have less protein overall, the pattern of both -/+ IPTG lanes are identical. There appears to be no induction.