Matt Gethers/CRI, Thailand/Labwork/Generating the HmgR Mutant/Week of 8.11.08

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8.12.08

I doubt I'll have a chance to transform the Lox/Gentamicin cassette into Pseudomonas, but I can at least try to finish generating the cassette for HmgA.

To Do:

Summary:

I transformed the pKn005.L3 and L4 ligation products into DH5α. Hopefully will get colonies tomorrow.

8.13.08

As of 0800 this morning, there are no colonies on any of the plates from yesterday's transformation. Looks like this cloning step just wants to be difficult. There's not really much else I can do here.

Before I leave today, submit sequencing for both pKn006 clones.

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