Matt Gethers/CRI, Thailand/Labwork/Digests/NdeI and BamHI Double Digest/8.5.08 Gel Results
8.5.08 Digest Gel
|Lane 1||Lane 2||Lane 3||Lane 4||Lane 5||Lane 6|
|5 μl λ maker||pET-11a no enzymes||pET-11a NdeI||pET-11a BamHI||pET-11a BamHI/NdeI||HmgR ORF BamHI/NdeI|
120 V for 30 minutes, 1% gel, stain for 15 minutes in EtBr. Lanes 3, 4, and 5 all differed from lane 2 suggesting that the enzymes are working properly and that the double digest is in fact yielding a doubly cut vector. I excised the ~5 kb band from lane 5 and the ~800 bp band from lane 6. Gel purified, eluted in 50 μl elution buffer.