Matt Gethers/CRI, Thailand/Labwork/Digests/HincII

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Contents

HincII

Reaction Mix

Reagent Volume
Template 10 μl
HincII 0.5 μl
Fast Digest Buffer2 μl
H207.5 μl
Total 20 μl

Reaction Conditions

Incubate mixture at 37oC for 30 minutes and inactivate at 65oC for 15 minutes on the heat block.

Run Notes

7.4.08

Ran digest on pKn001.P1 using only 5 μl of template and 12.5 μl of water. Other reagents were as written. Incubated at 37oC for an hour, room temp for ~20 minutes, 65oC inactivation for 15 minutes.

7.10.08

Ran digest on pKn001.P1 again using only 5 μl of template and 12.5 μl of water. Other reagents were as written. Incubated at 37oC for an hour, room temp for ~20 minutes, 65oC inactivation for 15 minutes.

References

Fast Digest HincII

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