Matt Gethers/CRI, Thailand/Labwork/Digests/BamHI

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Contents

BamHI

Reaction Mix

Reagent Volume
Template 10 μl
BamHI 0.5 μl
Fast Digest Buffer2 μl
H207.5 μl
Total 20 μl

Reaction Conditions

Incubate mixture at 37oC for 30 minutes and inactivate at 65oC for 15 minutes on the heat block.

Run Notes

7.29.08

Used BamHI to digest pKn003.P1. Used 5 μl pKn003, 0.5 μl enzyme, 2 μl buffer, and 12.5 μl water. Incubated for 1 hour 15 minutes at 37 degrees and inactivated at 65 degrees for 15 minutes. Put in 4 degree over night.


8.12.08

Used BamHI to digest more pKn003.P1. Used 5 μl pKn003, 0.5 μl enzyme, 2 μl buffer, and 12.5 μl water. Incubated for 30 minutes at 37 degrees and inactivated at 65 degrees for 15 minutes.

References

Fast Digest BamHI

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