MSUSBC Lignin

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MSUSBC Lignin Project

Background

Pardon our Progress

Papers

  1. On the Interaction of Lignin Peroxidase with Lignin
  2. Organization and Differential Regulation of a Cluster of Lignin Peroxidase Genes of Phanerochaete chrysosporium

"Why P. chrysosporium maintains multiple isozymes to catalyze presumably similar reactions remains unclear. Some substrate and kinetic differences between LiP isozymes have been observed previously (14, 20) and may indicate specific roles for individual LiPs during lignin depolymerization. Alternatively, it is possible that the majority of LiPs are redundant, having arisen through various chromosomal rearrangements such as duplications, translocations, or unequal crossover events during meiosis. The report of an insertion element that transcriptionally inactivates lipI2 indicates that not all alleles are necessary for efficient lignin depolymerization (17). In addition, there is growing evidence that redundant genes are maintained if they are not deleterious to the organism (18, 39)."

  1. cAMP-Mediated Differential Regulation of Lignin Peroxidase and Manganese-Dependent Peroxidase Production in the White-Rot Basidiomycete Phanerochaete chrysosporium

"LIP and MNP gene expression appears to be differentially regulated depending on the intracellular concentration of cAMP. These results show that cAMP plays a key role in the regulation of production of LIPs and MNPs in P. chrysosporium."

  1. Ligninolysis by a Purified Lignin Peroxidase

LiP definitely plays a major role in the initiation of Ligninolysis. The enzyme alone is capable of breaking the lignin structure, but repolymerization occurs when the proper mechanisms are not present to uptake the polymer units. Veratryl alcohol was also noted to be of high significance to LiP activity.

  1. Biosynthetic Pathway for Veratryl Alcohol in the Ligninolytic Fungus Phanerochaete chrysosporium

This one shows the pathway for Valc synthesis in white rot fungi.

Wood Products Meeting (4/25)

  • 10yr pine showed significantly different PC results upon treatment with two different organisms.
  • Brown Rot(GT7), White Rot(PC)
  • I don't know if this info is of any significance to us because we are only interested in the uses of white rot, and it is pretty clear that white and brown rot are significantly different.
  • Deterioration was not measured to any significance.
  • It would be awesome if we could actually design an entire process using our project. This would be great for the competition, not to mention our engineering skills.
  • Mature wood is a mix of pine lumber
  • Dr. Diehl has dry mass data on the actual amount of degradation.
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