Kubke Lab:Research/CND/Records2010-2011Summer/RC013
| Cranial Nerve Development | Experiment |
Embryo details
Species: Gallus gallus domesticus
Embryo Name: RC013
Embryo stage: ST30
Staging description: Staged by Reuben according to the Hamburger and Hamilton (1951) staging system. (Notebook entry 10th January 2011). Staging has not been confirmed.
- Shape of beak matches ST30.
- There is a distinct dip in the hindbrain's curve which is present on ST29-ST30 embryos.
- The forebrain is split along the midline running laong the rostro-caudal axis.
- There are no dots in the eye only faint pigmentation around the perimeter (<ST31).
- Morphology of the toes matches 30 more closely than 29.
Fixation: PFA
Cryoprotection: None.
Material label and storage: Stored in vial filled with PFA labeled RC-013 and placed in a container labeled 'Reuben's staged embryos' next to the dissection microscope.
Experiment details
Objective:To use as a practice embryo for staging.
Procedure: Staged the embryo by observing the embryo under a dissection microscope and comparing its morphological properties with those described in the Hamburger and Hamilton (1951) paper whilst looking at our own compilation of images organized by stage.
Comments:
Results
Embryo is approximately ST30.
Images
Summary
This embryo may be used in further experiments as approximately a ST30 embryo however confirmation is needed.
