Kubke Lab:Research/CND/Records2010-2011Summer/RC010
Cranial Nerve Development | Experiment |
Embryo details
Species: Gallus gallus domesticus
Embryo Name: RC010
Embryo stage: ST25
Staging description: Staged by Reuben according to the Hamburger and Hamilton (1951) staging system.
(Notebook entry 10th January 2011 ). Staging has not been confirmed.
- Too grooves present but not distinct (<ST26).
- Tail region segmented.
- Visceral clefts still visible (<ST26).
- Shape of the hindbrain has a sligh groove which resembles the images for ST25.
Fixation: PFA
Cryoprotection: None
Material label and storage: Stored in vial filled with PFA labeled RC-010 and placed in a container labeled 'Reuben's staged embryos' next to the dissection microscope.
Experiment details
Objective: To use as a practice embryo for staging.
Procedure: Staged the embryo by observing the embryo under a dissection microscope and comparing its morphological properties with those described in the Hamburger and Hamilton (1951) paper whilst looking at our own compilation of images organized by stage.
Comments:
Results
Embryo is approximately ST25.
Images
Summary
This embryo may be used in further experiments as approximately a ST25 embryo however confirmation is needed.