Kubke Lab:Research/CND/Records/NF020
nf020 - chicken embryo sb - Stage 26 - 2006.10.02
Experiment
Objective Inject anterior vs posterior branch of VII to see if it labels different groups of MNs.
Procedure
- Left Anterior VII DiO (may be some DiO on ganglion)
- Left Posterior VII DiI
- Right Anterior VII DiO
- Right Posterior VII DiI
Notes
Not happy with injections
Files
- nf020_Series003
- nf020_Series004
- nf020_Series010
- nf020_Series011
- nf020_Series019
- nf020_Series020
Results
Caudal Facial
- Afferents
Clearly projecting both caudally and rostrally. Rostral branch seems to be in a somewhat more lateral position, except for what appears to be a very thin bundle of a few axons on the lateral-most edge of the wholemount on the caudal side. Caudal bundle appears in a more medial position, except for a few thin and tightly packed axons on the more lateral side. Only got afferents on injections on one of the sides
- Efferents
Caudal branch clearly labels more superficial/ medial column of mn on both r4 and r5, but primarily on R4. The more lateral deep colum is also well labelled.
- Other
Profiles on the floor plate - few
Rostral Facial
Injection on one side had a bit of spill into the ganglion, and on that side, DiO seems to have labelled everything VII.
- Afferents
Looks like the rostral afferents labelled from rostral facial form a rather defasciulated rostral branch that seems to stop short probably near the edge of r4/r3. The caudal branch clearly has 2 branches, with the lateral seeming more defasciculated, but neither of then forms a tight bundle.
- Efferents
The deep/lateral motoneuron column seems to be more intensely labelled in r5, although there might be a bit of labelling in r4. The more superficial/medial group is labelled in both r4 and r5, but not as much as with DiI from the rostral branch. There is in fact very little labelling.
- Other
Summary
Some slight differences in the motoneuron pattern of labelling - it looks like caudal contributing more to motoneurons whereas rostral contributes more to afferents.
Questions
Future