Kubke Lab:Research/CND/Records/HK030

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EmbryoName HK030- chicken embryo- Stage 20 - 12.1.15

Experiment

Objective To dye seperate branches of each trigeminal nerve different colours to follow their cell bodies and see how they are organised. Also the dye each side of the same nerve a seperate colour so that I can notice any decussation or cross talk at the floorplate.

Procedure

  • Trigeminal nerve L- Ant branch DiO and post branch DiI, opp on R side


Notes nerves were cleanly dissected and did a light sprinkle of dyes on each stub, by the time I looked (4hours post-incubation) I noticed that the stub was totally dyed and the dye had obviously transported including the DIO, just not into the hindbrain

Files

Results

V

(green and orange)

Anterior Branch L side

  • Afferents

DiO DIDN"T LABEL

  • Efferents


  • Other

Posterior Branch L side

  • Afferents


  • Efferents


  • Other


Anterior Branch R side

  • Afferents



  • Efferents

Why did I get images of efferents in DII on the R side?? there aren't technically supposed to be efferents as part of the V1 nerve as it is purely sensory

  • Other

Posterior Branch R side

  • Afferents

DiO DIDN"T LABEL

  • Efferents


  • Other

Summary

Questions could there actually be a motor component of V1? or do the efferents dye shortly after realising they are in the wrong place

Future

note how many times I image efferents through dying V1 and see if it occurs a convincing amount of times to start addressing the question of why there are there

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