Kubke Lab:Research/CND/Records/HK024
From OpenWetWare
Jump to navigationJump to search
EmbryoName HK024- chicken embryo- Stage 18 - 9.12.14
Experiment
Objective To replicate the dye injection of HK023 but in a slightly younger embryo Dyes of different colours on Trigeminal (DiO) vs 7/8th nerve complex (DiI) to see interactions between efferents of one nerve and afferents of other. Also to practice the injection on a younger chicken (slowly moving down to stage 18-17)
Procedure
- Trigeminal nerve bilaterally DiO crystals
- 7th/8th nerve bilaterally DiI crystals
Notes Slight contamination that I tried to remove on the R side of the 7/8th nerve complex. Other than that a good injection, put more on 7/8th nerves than the trigeminals because I got less dye in HK023 so am trying to do the same here.
Files
Results
Facial
(Green)
- Afferents
- Efferents
VIII
(green)
- Afferents
- Efferents
- Other
Basal plate
(red)
Summary
Questions
Future