Kubke Lab:CND/MH006
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Cranial Nerve Development | Experiment |
Embryo details
Species:
Embryo Name: MH006
Embryo stage: 28
Staging description: Eye: Midbrain ratio, mandibular arch and limb development
Cryoprotection: Yes
Experiment details
Objective:To determine what are the best parameters to cut a stage 28 embryo.
Procedure:
Cryostat Sectioning Cryostat settings (for a more detailed protocol visit Kubke_Lab:Cryomicrotomy)
Cryostat | Histology Lab |
Knife | |
Day Cut | |
Knife Angle | Close to 0 (0.3-0.5) |
Chamber Temp | -17 C |
Object Temp | -17C |
Glass Slides | Gelatin subbed |
Plane of section | {{{Plane of section}}} |
Number of slides | |
Observations |
(Include in your observations, eg ,were the sections serial, was any section lost, was quality assessed, etc)
Cresyl Violet staining
For more informattion see Kubke_Lab:Nissl_Stain_Protocol
Solution | Time | Comments |
---|---|---|
Defatting and rehydration step | ||
Water | ||
75% alcohol | ||
95% alcohol | ||
100% alcohol 1 | ||
100% alcohol 2 | ||
Xylene 1 | ||
Xylene 2 | ||
Xylene 3 | ||
Xylene 2 | ||
Xylene 1 | ||
100% alcohol 2 | ||
100% alcohol 1 | ||
95% alcohol | ||
75% alcohol | ||
Water | ||
Staining and differentiation step | ||
Cresyl Violet | ||
50% alcohol | ||
70% alcohol acetic acid | ||
95% alcohol | ||
100% alcohol 1 | ||
100% alcohol 2 | ||
Xylene 1 | ||
Xylene 2 | ||
Xylene 3 | ||
Coverslip |
Comments: