Kubke Lab:/Notebook/Cranial nerve development/2015/01/08

Cranial Nerve Development

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Entry title General Helen Things just weren't flowing the way they normally do for me today. I felt frustrated with my dissection, the clearing took ages and I had my embryo ready at 2.30, and by that time it seemed the dye had faded and I basically couldn't even see an image. I don't know what it is but my injections are just not working well. Especially compared to the last two that I did before the break that were so beautiful and clear. The dyes now are just faded and fuzzy, even if I clear them for exactly the same time, if not even slightly longer. I am getting very frustrated and need a good embryo to refocus me because right now it feels like getting a project done is a very long long way off and it almost feels like I am starting from scratch. Fabiana