Koch Lab:Protocols/PEM (BRB80) from Cytoskeleton
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BRB80 is the same thing as PEM. PEM stands for "PIPES, EGTA, Magnesesium." BRB stands for either "Borisy" or "Brinkley" Resuspension Buffer. "80" stands for 80 mM PIPES.
Steve Koch 17:06, 16 June 2009 (EDT): As of now, we're using a 10 mL stock from Cytoskeleton.