Koch Lab:Inventory/BRB80 (PEM)

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BRB80 or PEM (PIPES, EGTA, Magnesium) buffer

Desired: 80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, pH 6.9

• This is the page for the buffer that Andy, Brigette and Linh made on 19:07, 22 June 2009 (EDT)
• About 205ml were created\
• Concentrations:
  • 1mM MgCl₂
  • 1mM EGTA
  • 80mM PIPES
  • 12.5mM NaOH
• New batch made 8/28/2009
• Just over 200mL created
  • 80mM PIPES
  • 1mM EGTA
  • 1mM MgCl₂
  • 12.9mM NaOH

References

• Cytoskeleton PEM recipe
• The actual recipe Steve used at Sandia
• Recipe on kinesin home page (almost the same as cytoskeleton, but slightly lower pH)
• FriendFeed thread: what does the acronym BRB80 stand for?