Knight:Synthetic promoter design

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Notes on results of various synthetic promoter designs.

Sequences

Heterodimers:
                         -35                    -10
Promoter1 cacgtgtgcgtgggTTGACAcgtgtgcgtgggaagtcGATACTgagcaca
Promoter2               TTGACAcgtgtgcgtgggaagtcGATACTtagattcacgtgtgcgtggg
Promoter3 cacgtgtgcgtgggTTGACAcgtgtgcgtgggaagtcGATACTtagattcacgtgtgcgtggg
Promoter4 cacgtgtgcgtgggTTGACAcacgtgtgcgtgggaatGATACTgagcaca
Promoter5               TTGACAcacgtgtgcgtgggaatGATACTtagattcacgtgtgcgtggg
Promoter6 cacgtgtgcgtgggTTGACAcacgtgtgcgtgggaatGATACTtagattcacgtgtgcgtggg
Homodimers:
                            -35                   -10
BBa_R2000         agtttattcTTGACAtggtcccacgcgcgtggGATACTacgtcag
BBa_R2001         agtttattcTTGACAtggtcatattacggtgaGATACTcccacgcgcgtggg
BBa_R2002         agtttattcTTGACAtggtcccacgcgcgtggGATACTcccacgcgcgtggg
Promoter8  tttatcaaaaagagtgTTGACAtttttaagtcccacgcgTGGGATtagattcataaatttgagagaggagtt
Promoter10 tttatcaaaaagagtgTTGACAtttttaagctcccacgcgTGGGTtagattcataaatttgagagaggagtt
Promoter12 tttatcaaaaagagtgTTGACAtttttaatcccacgcgTGGGAATtagattcataaatttgagagaggagtt
Promoter9  tttatcaaaaagagtgTTGATCccacgcgtgggatatagGATACTtagattcataaatttgagagaggagtt
Promoter11 tttatcaaaaagagtgTTGACTCccacgcgtgggaatagGATACTtagattcataaatttgagagaggagtt
Promoter13 tttatcaaaaagagtgTTGTCccacgcgtgggactatagGATACTtagattcataaatttgagagaggagtt

Results

Name Synthesized (Issues) Promotes (Strength) Represses
Promoter1
Promoter2
Promoter3
Promoter4
Promoter5
Promoter6
Promoter7
BBa_R2000
BBa_R2001
BBa_R2002
Promoter8
Promoter9
Promoter10
Promoter11
Promoter12
Promoter13

Contact

Reshma Shetty

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