Klapperich Lab:Notebook/Lab Meeting Notes/2009/05/12

Project name

Main project page Previous entry      Next entry

12 May 2009 Lab Meeting † Announcements SIGN UP FOR NEW LAB DUTIES WITH SONALI. New lab meeting time T 10a-12pm room 705 starting 5/12/2009 PDMS Fixture holder - make more at machine shop. Follow proper cleaning procedures with PU molds. † Flu R01 RNA extraction troubleshooting. Mass Spec of elution? Martin Steffen? Bioanalyzer not working.. why? LOD - Serially dilute our samples and look to see if we can see it when we dilute the contaminants and the DNA/RNA Absolute Quantification assays FTIR-ATR look at the eluant? † Coulter Flu Fraunhofer Project Fraun folks coming to get trained next week. RNA isolation. IBC for Fraun flu. in process. † SEPSIS Project Revision in process due 5/20. MM to CMK at the end of this week. †RNA project Jeff visiting week of June 1st † COBRA Part last week was leaking b/c of flatness issues. No SERS test. Mask our for SU-8 mold. (PDMS) Test again before next Wed. Evap system (JD and JZ) Let's get this to a point where we can publish and move on paper 1: Evap with Sol Gel substrate. paper 2: Covap with PMA, Rhodamine, PS beads?...virus...? † Virus concentration ATCC flu virus grew and harvested Plaque assay done. † Valve Array/Valve building - done. SP students are gone? Alex. JD. † Fraunhofer: LOAC Paper in revision Yeah! Bonding meeting with MIT folks - Alex left some samples with them. Updates? † Biointerfaces group Final draft with collaborators. designing herringbone groove for better trapping (Mask writer down until next Tuesday) investigate the stability under different Peclet numbers (ratio of hydrodynamic force and Brownian force). † CIMIT- Colson Grant IRB still in revision. † PCR repeated test of the PCR efficiency for different designs of channel. test PCR efficiency using smaller DNA size (63bp product). analyze PCR product for different temperature using bioanalyzer. getting statistical data for the activation energy from 50 particle simulation (Fig. 5-7) Mincheol has written the introduction, results and discussion, and conclusion. will develop large scale particle modeling in full PCR model (30 PCR cycles or 20 PCR cycles) † RCA/HDA Giving up on white paper. Think about other grant outlets. Preliminary no-valve design for 25 microliters. 20 more test chips to Sonali (AACC poster). Sonali try to do 10 microliters in the tube * Updates? Jane is working on passive valve characterization: threshold pressure not related to cantilever length or bonding time, try hole size next † Silica Optimization (Lambda): Run single PCR product from QQ or Sonali the test with the bioanalyzer