Klapperich Lab:Notebook/Lab Meeting Notes/2009/04/02

From OpenWetWare

Jump to: navigation, search
Project name Main project page
Previous entry      Next entry

2 April 2009 Lab Meeting

Pressure sensor - quoted $325 SP70A or $1000+ SP70D, returning old one for them to look

  • Co. says that it works fine. Transducer up to 1000 psi. We are getting it back.

STATUS?

† Announcements

  • Need posters for Oakridge soon.


† Flu R01



† SEPSIS Project

  • Submitted to LOC.


†RNA project

  • Need to get Jurkat RNA to Jeff. He may visit in May.


† COBRA

  • Integration update? Let's get some of this done before JD leaves for Korea.


  • Substrates (JZ)
    • Let's get this to a point where we can publish and move on....JZ and CMK to discuss.

- nanopatterned COP provides access to repeatable morphology
- Engineered COP substrate? UPDATE?

  • three categories of making new sbustrates 1) ebeam COP film, 2) COP hot eboss EVAP (Luca), 3)stamping/other intermediate method(Bjoern similar). (JD)


  • Colloid experiment.

- Do Rhodamine.

    • ordered

-Need a size specific positive control. PS beads? charged?

    • 1um unmodified, undiluted PS beads give distinctive spectrum on Ranjith's substrate
    • co-evaporating diluted PS beads didn't give a signal: difficulty in locating concentrated particles


† Valve Array/Valve building - done.


† Fraunhofer: LOAC

  • Paper submitted to LOC


† Biointerfaces group

  • Cathie in charge of Bionterfaces Paper1. Cathie has final draft now before it goes to collaborators.
  • designing herringbone groove for better trapping
  • investigate the stability under different Peclet numbers (ratio of hydrodynamic force and Brownian force).


† CIMIT- Colson Grant

  • IRB still in revision.


† PCR

  • Cathie read and gave back PCR 1 paper to QQ and MCK
  • Qingqing has written the introduction
  • repeated test of the PCR efficiency for different designs of channel.
  • test PCR efficiency using smaller DNA size (63bp product)
  • analyze PCR product for different temperature using bioanalyzer.
  • will make figures for PCR1 paper.
  • developing large scale particle modeling in full PCR model (30 PCR cycles or 20 PCR cycles)


† RCA/HDA

  • Cathie submitted One pager Whitepaper. Waiting for response.
  • convert this whitepaper to grant? RCA/HDA on a chip for NIH...anyone interested? ANYONE?
  • Preliminary no-valve design for 25 microliters.
  • 20 more test chips to Sonali (AACC poster).
  • Sonali try to do 10 microliters in the tube


† Senior project

  • Megan
  • Test on Thu, Fri worked but failure after some cycles.
  • Need to enhance bonding (e.g. Ar or O2 ashing)


† F31: Cochlea



† Silica Optimization (Lambda):

  • The following will be submitted monday


  • total of 20 channels 10 as negative and 10 positive with 100ng total NA mass. Emission spectrum of negative samples with excitation at 488nm.
  • Emission spectrum of negative samples at excitations corresponding to the NA sampler kit dyes
  • Emission spectrum for the positive samples split into 2 groups , one with pico green and the other without at 488nm .
  • Chip ran with total of 5ng of NA , pico green using the low range standard curve



Personal tools