Klapperich Lab:Notebook/Lab Meeting Notes/2009/02/12

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12 February 2009 Lab Meeting Agenda Status: compressed air and vacuum? Why did the work not happen? † Announcements Sonali out for a week. New senior project team. MicroTAS abstracts. † Flu R01 Modified Schedule this week. Plaque Assay. † SEPSIS Project Sonali done with draft. . †RNA project More formal report to Jeff from Cathie. Do cDNA prep. Spec, gels, send to Jeff B. Shipping? † COBRA Mark- Control circuit - done, need to be tested. Integration test from here forward br> - Direct measurement with engineered SERS substrate. Test with engineered SERS substrates - 500umx500umx50um dimension informed to Luca and Bjoern - Need to schedule when they are ready. Substrates (JZ) - Try glass fixture. - - Colloid experiment. inconclusive. Look at SEMs - difficulty in locating the bacteria - needs much higher particle/bacteria ratio † Valve Array/Valve building (FJ) Teddy on track. Valve tested † Fraunhofer: LOAC Paper drafting! † Biointerfaces group Final experiments in process? Need one more experiment next week † CIMIT- Colson Grant IRB still in revision. † PCR flu cDNA　ｗａｓ　ｓｕｃｃｅｓｓｆｕｌｌｙ　ａｍｐｌｉｆｉｅｄ　ｏｎ　ｃｈｉｐ，ｏｎｅ　ｂａｎｄ　ｓｈｏｗ　ｕｐ　ｉｎ　ｇｅｌ．　ｅｘｐｅｒｉｍｅｎｔ　ｈａｓ　ｂｅｅｎ　ｒｅｐｅａｔｅｄ　ｆｏｒ　ｔｈｒｅｅ　ｔｉｍｅ． ｗｏｒｋｉｎｇ　ｏｎ　ｔｈｅ　surface coating of zeonex, looking for an effective way to verify the PEG layer tried contact angle, device broken SEM did not give out a lot of information scheduling XPS DNA chain model is being made (5 beads model tested). † RCA Cathie submitted One pager Whitepaper. Waiting for response. † HDA Large volume filling and valve tested. Design issue - whole integration or assemble parts (SPE & HDA chamber) † Senior project Megan 1)did some preliminary bonding with Zeonex and teflon film. 2) emailed off the Mask Design to FineLine † F31: Cochlea Pre-gent vs. Post-gent-- most interesting data: decorin upregulation, fibronectin gradient Six antibodies. Verifying 4 with protein pre- and co-incubation with antibody. Could not find the other two proteins Finalized poster and printing for ARO ARO is next week † Silica Optimization (Lambda): Overview to look at the data collectively [1] Next E. coli. try another color DNA dye. Pico green works ok http://www.invitrogen.com/site/us/en/home/References/Molecular-Probes-The-Handbook/Nucleic-Acid-Detection-and-Genomics-Technology/Nucleic-Acid-Stains.html Specifically buy http://products.invitrogen.com:80/ivgn/en/US/adirect/invitrogen?cmd=catProductDetail&entryPoint=adirect&productID=N7565&messageType=catProductDetail