Klapperich Lab:Notebook/Lab Meeting Notes/2008/12/16

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16 December 2008 Lab Meeting Agenda Check with B&G about compressed air and vacuum? † Announcements No meeting on 12/23 or 12/30 Meeting Time for Spring Semester is THURSDAYS 3-5pm ROOM 705. Stating 1/8/09. See Lab Calendar for dates. † Flu R01 First samples? Plaque Assay. Alex needs to be trained to make SU molds. Trained to make the monoliths (by Hussam By 1/15/09). † SEPSIS Project Sonali working on first draft of new version paper by 12/25. Journal? Hussam working on Lambda phage data. †RNA project Report to Jeff from Cathie. Do cDNA prep. Spec, gels, send to Jeff B. Shipping? † COBRA Mark- Control circuit - This will be done by January. Evap quantification experiments ongoing. Still working on live/dead assay.(JD, JZ) - problems: dead but green in the end - called tech support. - tests: supernatant fluorescent? excessive dye to observe time-lapse change in green/red ratio. - omit washing step before dilution: wash, dye, incubate, dilute, instead of wash, dye, incubate, wash, dilute - try running the experiment first, then running the live/dead dye through second. Substrates (JZ) - new set made, in glove box: the 1st 3 on the DOE. SERS signal strong, SEM today DOE for the experiment. - need to add humidity as it changes even in the glove box Colloid experiment. inconclusive. Look at SEMs - difficulty in locating the bacteria - needs much higher particle/bacteria ratio - experiments outside the channel first? Prospectus framework/discussion: target the end of this week 1/23/09. † Valve Array/Valve building (FJ) Turn over platform to Teddy TEDDY needs to meet with Mark. † Fraunhofer: LOAC So so close! † Biointerfaces group Strobe experiments? Jason cannot do them, perhaps MCK can. MCK will talk to Jason this week. Made new design for trapper. Mask writer was broken. † CIMIT- Colson Grant IRB still going/ revisions by tomorrow to Ian. Target date 1/1/09 STAR-CD Renewal - January ($2K) † PCR Improve the gel concentration 1.2% agarose. See if you can separate out primer dimers. UPDATE? Look into Melting temp analysis on the 7300 - Done. what is contaminated? Only 1/5 chips are good at the hot embossing step. The vaccum chuck will be checked and o-rings changed - helped a bit. ON HOLD * Fixuture for PCR chip. The heater and the thermocouples would be on the fixture. ON HOLD Try microscope for looking a fluor. Learn how to look at the data in Image J 52, 60, 65 C repeats. † RCA Cathie submitted One pager Whitepaper. Waiting for response. † Senior project Megan 1)did some preliminary bonding with Zeonex and teflon film. 2) emailed off the Mask Design to FineLine † F31: Cochlea Set up adviser meeting Jan Post Gent vs. Pre gent - 4 proteins. 3/4 no change. † Silica Optimization (Lambda): Update by 12/24. Do three elutions. Next E. coli.