Julius B. Lucks/Bibliography/Gottesman-MicrobiolMolBiolRev-68-2004

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Notes on [1]

  • review of history of discovery of lambda - what it was used for in the field
  • establishment of repression and prophage insertion are distinct steps in establlishment of lysogeny

Prophage Insertion

  • Cambell mechanism - 1968 review (28) - Fig 1
    • also (24,31)
    • Hershey - cohesive ends making ring (91) - also (207)
      • DNA ligase helps ring closure - Gellert (68)
    • phage-encoded site-specific recombinase (178,179)
      • see also (87,138) for attP-attB recombination rules - site-specificity gives high efficiency and does not require extensive homology
  • (118) - 15 bp region where recombination occurs - see also 200
  • Guerrini (87) - prophage excision not simple chemical reversal of prophage insertion
    • lambbda mutants unable to excise (84)
      • (49) - diff conditions for favoring integration over excision and vice versa
      • integrase more stable than Xis (201)
    • Xis produced from P_L, Int produced from P_Int
      • P_Int activated by CII - CII coordinates Int synthesis and repressor synthesis (35)
    • after induction, both Int and Xis produced from P_L
      • (49,83) - Guarneros and Court - how Int produced from both P_L and P_Int - Fig 2
        • sib - sit of endonucleolytic RNase cleavage - promoter distal to int
        • N - suppresses termination of certain phage transcripts
  • P_L transcripts contain sib because termination suppressed by N - therefore cleaved and destabilizes int message
    • P_L transcript made after induction does not contain sib b.c. of gene permutation after inserts (Fig 1)
  • P_Int transcripts terminate before sib and are more stable (173,174)
    • start site is within xis
    • terminate at T_Int, and do not contain complete sib
  • excision requires Int and Xis
    • in vitro integration/excision - (77,148,149)
  • Int a type I topoisomerase (115)
  • IHF (host integration factor) required for integration (150,151)
    • 2 subunits - ihfA (hid/himA), and ihfB (hip/himD) (114,136,204)
    • architectural role - bends DNA to form Int-attP-IHF intasome (73)
  • effective integration requires negative supercoils (69,139)

References

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  1. Error fetching PMID 15590784: [Gottesman-MicrobiolMolBiolRev-68-2004]
    Notes

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