Jimenez-gomez lab:stock antibiotics

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Institute Jean Pierre Bourgin
INRA - Versailles
Route de Saint Cyr
78026 Versailles Cedex
France
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Old address:
Max Planck Institute for Plant Breeding Research
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Antibiotic stocks

abbstock conc.LB plate
conc.
Vol. in 500ml
LB agar
MS plate
conc
Vol. in
1L MS
ampicilin ap50 mg/ml100 μg/ml1 ml
kanamycin km/kn50 mg/ml50 μg/ml0.5 ml50 μg/ml1 ml
spetinomycin sp100 mg/ml100 μg/ml0.5 ml
chloramphenicol cm34 mg/ml in ethanol34 μg/ml0.5 ml
rifampicin rif34 mg/ml in methanol150 μg/ml2.2 ml
tetracycline tc12 mg/ml12 μg/ml0.5 ml
hygromycin hyg20 mg/ml 20 μg/ml1 ml
carbenicillin carb100 mg/ml 500 μg/ml5 ml
thiamine 50 mg/ml 50 μg/ml1 ml
gentamycin* genteffective dose 6.5 mg/ml15 μg/ml0.75 ml75 μg/ml1 ml
basta 20 mg/ml 20 μg/ml1 ml
zeocin Zeo100 mg/ml25 μg/ml0.125 ml
* the potency of gentamycin varies from stock to stock, the volume added must be calculated according to |-
For pPZP211 Spectinomycin Dihydrochloride and Streptomycin sulfate disolved in dd water with a final concentration of 30 mg/ml each. Take 1 ul for each mL of medium (final concentration of 30 ug/mL.
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